An Experiment to look for the Rate of Result of Succinate to Fumerate

Introduction:

In Eukaryotic cells the mitochondria may be the organelle specializing in strength extraction. This extraction takes place in an activity referred to as cellular respiration. In cellular respiration sugars, essential fatty acids and proteins are divided into skin tightening and, water and cellular energy called adenosine triphosphate or ATP. During cellular respiration almost all of the ATP is generated through electron transportation in the mitochondrial matrix. However, there exists a very important enzyme that's located beyond the mitochondrial matrix. This enzyme is usually succinate dehydrogenase and its own coenzyme, flavin adenine dinucleotide or FAD. Both of these enzymes together are in charge of the oxidation of succinate to fumarate in the KrebРІР‚в„ўs cycle part of cellular respiration, as observed in Figure 1.

Figure 1: Kreb's Cycle Diagram (http://ccnmtl.columbia.edu/projects/biology/lecture9/krebs.htm)

To

determine the charge of result of succinate to fumerate the reduction

of an artificial electron acceptor is normally monitored and measured

spectrophotometrically. To employ an artificial electron acceptor the

normal route of electrons should be blocked. A poison known as sodium

azide is utilized to do this result.